Generalists and specialists decomposing labile and aromatic biochar compounds and sequestering carbon in soil

Biochar plays an important role in soil carbon (C) sequestration, while the effectiveness of sequestration is mostly determined by microbial preferences to utilize different biochar components (i.e. labile substances or fused aromatic backbone). Thus, it investigate responses bacterial community and specific taxa, associated C metabolic functions easily mineralizable C) backbone persistent C). Here, we separated into backbone, used 16S sequencing conjunction with addition internal standard quantify absolute copy number various taxa. The decreased quotient 47 %, incorporating more biomass contributing rather than releasing atmosphere, caused opposite trends. generalists utilizing were mainly from Oxalobacteraceae (Proteobacteria) Sporosarcina (Firmicutes). specialists responsive for Myxococcaceae Flavisolibacter, those Ktedonobacteraceae Bradyrhizobiaceae. Microbial abundance was 3.3 times larger indicating energy sources slow utilization versatile habitats. Our work revealed strategies organic very contrast availability, their contributions depending on components.